DeutschesFachbuch.de : Peptide Characterization and Application Protocols

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Contents
Prefacev
Contributorsix
PARTI:CHARACTERIZATION
1.HPLC Analysis and Purification of Peptides
Colin T. Mant, Yuxin Chen, Zhe Yan, Traian V. Popa, James M. Kovacs, Jan...
2.Identification of Proteins Based on MS/MS Spectra and Location of Posttra...
Kathryn L. Stone, Myron Crawford, Walter McMurray, Nancy Williams, and Kenn...
3.Determination of Phosphorylated and O-Glycosylated Sites by Chemical Target...
Gary M. Hathaway 79
4.Synthesis, Biosynthesis, and Characterization of Transmembrane Domains of a...
Fred Naider 95
PARTII:APPLICATIONS
5.Application of Topologicaly Constrained Mini-Proteins as Ligands, Substrates...
Janelle L. Lauer-Fields, Dmitriy Minond, Keith Brew, and Gregg B. Fields 12...
6.Proteolytic Profiling of the Extracellular Matrix Degradome
Diane Baronas-Lowell, Janelle L. Lauer-Fields, Mohammad Al-Ghoul, and Gregg ...
7.ß-Amyloid Protein Aggregation
Marcus A. Etienne, Nadia J. Edwin, Jed P. Aucoin, Paul S. Russo, Robin L...
8.Radiometal-Labeled Somatostatin Analogs for Applications in Cancer Imaging ...
Jason S. Lewis and Carolyn J. Anderson 227
9.Cell-Penetrating Proline-Rich Peptidomimetics
Josep Farrera-Sinfreu, Ernest Giralt, Miriam Royo, and Fernando Albericio 241
10.Peptide-Mediated Targeting of Liposomes to Tumor Cells
Evonne M. Rezler, David R. Khan, Raymond Tu, Matthew Tirrell, and Gregg B...
11.Peptide-Mediated Delivery of Nucleic Acids into Mammalian Cells
Sébastien Deshayes, Federica Simeoni, May C. Morris, Gilles Divita, and...
12.Broth Microdilution Antibacterial Assay of Peptides
Laszlo Otvos and Mare Cudic 309
13.Depsipeptide Synthesis
Maciej Stawikowski and Predrag Cudic 321
Index 341
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Index
AActivity-based protein profiling (ABPP) 168
Affinity chromatography 138
a-factor receptor 96, 97
Alkaline phosphatase digestion 68
Amino acid side-chain hydrophilicity/hydrophobicity coefficients 6
Amino acid a-helical propensity values 7
Amino acid monoisotopic masses 66
Amino acid side-chain stability coefficients 9
Amphipathic a-helical peptide model 8
Anion-exchange chromatography (AEX) 10, 11
BBacteria 310, 317
ß-amyloid peptide 204, 205
- Aggregation 212
Bifunctional chelator 227, 230
Biosynthesis of membrane proteins
CCapillary electrophoresis 12, 14, 15, 37
Cation-exchange chromatography (CEX) 10, 11, 19
Cell delivery 260, 279, 280, 281, 300, 305, 306
Cell viability 206, 217
CD 44 139, 181, 182, 275, 283
Chelator-peptide Synthesis 229, 232
Circular dichroism (CD) spectroscopy
- Characteristic spectra 113, 114
- Membrane peptides 112
- ß-amyloid aggregation 212, 213
- Triple-helices 279, 284
Comparison of HILIC/CEX and RP-HPLC 33
Confocal laser scanning microscopy 262
Cytotoxicity 261
DDendrimers
- Functionalization 247, 257, 258
- Labeling 246
- Poly-proline synthesis 244, 245, 255
- Second generation 248
Depsipeptide 322, 323
Disulfide bonds
- Oxidation 142
- Stabilization 128
Dynamic light scattering 218, 223, 287
EEnzymatic stability of peptides 259
Ester bond formation
- Carbodiimide mediated 325
- N-hydroxysuccinimide ester mediated 327
- Mitsunobu reaction 328
- Yamaguchi reaction 329
Expression plasmid 103
FFlow cytometry 261
Fluorescence microscopy 280, 281, 284, 289
Fluorescence resonance energy transfer (FRET) 145, 175
- y -peptides
- y-amino-L-proline-derived 242
- Synthesis 253
GGlycosylation 80, 81, 151, 152
Growth inhibition 312
G protein-coupled receptor 96
HHydroxyl group protection 330
IImmobilized metal affinity chromatography (IMAC) 109
Integrin 139, 178, 181, 182, 270, 271, 274, 275, 283
Isotope-coded affinity tag (ICAT) 172, 173
LLiposome 270, 276
- Characterization 287
- Preparation 285
Lysine endopeptidase 81, 87
MMatrix-assisted laser desorption/ionization (MALDI) mass spectrometry Analysis of ph...
Matrix metalloproteinase (MMP) 130, 132, 174, 188, 272
- Signaling 181, 182
- Solid-phase assay 178, 192
- Substrates 146, 147, 148, 172, 175, 176, 192
Melanoma 138, 151, 152, 153, 153, 174, 179, 183, 186, 189
Membrane proteins Biosynthesis 99, 101
- Chemical synthesis 96, 98, 100
Mixed-mode hydrophilic interaction chromatography/cation-exchange chromatography (HIL...
OOligonucleotides 301
PPeptide-amphiphile 131, 177, 273, 274
- Cell-binding ligand 150
- Incorporation into liposomes 288
- Synthesis 133, 143, 282, 290
Peptide-based inhibitor
- Aggregation 204, 207, 208, 209, 210
- MMP 143, 144
Peptide drug carrier 301, 305
Peptide-oligonucleotide complexes 302
Positron emission tomography (PET) 228, 236
Phosphorylation 58, 67, 71, 80, 81
- ß-elimination 80, 81, 83
Preparative RP-HPLC of peptides and proteins 40
Proteolytic profiling 181, 182
RRadiolabeling 231, 233, 237
Radiometal 228, 229
Reaction monitoring 333
Receptor-binding assay 234
Reversed-phase high-performance liquid chromatography (RP-HPLC) 11, 14, 21, 111, 118,...
- Anionic ion-pairing 25
- Determination of instrinsic hydrophilicity/hydrophobicity 21
- Effect of temperature 27
RT-PCR 189, 191
SSarafotoxin 130, 131, 141, 143, 144
Scanning force microscopy 205, 213, 214
SDS PAGE 105, 193
siRNA 300, 303
Size-exclusion chromatography (SEC) 10, 13, 15
- Analysis of peptide oligomerization 17
Somatostatin 227
- Analogs 228
TTandem mass spectrometry (MS/MS) 57, 60, 61
- Collision energy 63
- De novo 64
- Peptide fragment ions 62
Targeted drug delivery 270, 277
Template-assembled synthetic proteins 126, 135, 155
Tissue inhibitor of metalloproteinase (TIMP) 130, 131, 132
Transmission electron microscopy 206, 214, 215
Triple-helical peptides 127, 273
Turbidity 310
WWestern blot 108
ZZymography 193
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